Figure 4.

Subcellular distribution of circadian proteins is modified by co-expression of Magel2. NIH3T3 cells were transfected with combinations of Xpress-Magel2 and circadian expression constructs. Transfected cells were scored as having the majority of the fluorescent signal either in the nucleus or in the cytoplasm. Differences in the nuclear/cytoplasmic distribution between transfections were detected using a Fisher exact test, with p < 0.05 deemed statistically significant. A) Example of primarily nuclear (N) or primarily cytoplasmic (C) localization of epitope tagged Bmal1 (in green, nucleus is in blue). B) Localization of Clock when expressed alone (C), expressed with Bmal1 (CB), expressed with Magel2 (CM) or expressed with Clock and Magel2 (CBM). C) Localization of Bmal1 when expressed alone (B), expressed with Clock (BC), expressed with Magel2 (BM) or expressed with Clock and Magel2 (BCM).